Bioanalysis and analytical chemistry
LC-MS/MS:
Triple quadrupole (Applied Biosystems): 1 APl-3200 and 3 API-4000
Triple quadrupole (Thermofischer): 1 TSQ Quantum Ultra
LC-UV-MS/MS-Radiodetector:
Triple quadrupole (Waters): 1 Quattro Micro coupled with:
- diode array UV detection (Waters 996 DAD)
- radioactive detection (Packard Flo One)
LC-UV-MS:
Single quadrupole (Waters). 1 ZMD coupled with a:
- diode array for UV detection (Agilent 1100 DA)
HPLC:
4 Alliance HPLC Systems (Waters)
- Diode array UV/visible detectors (Waters)
- Flo One Radiodetector (Packard)
- Qualified Empower system (Waters)
- Method Validation Manager
UPLC:
1 Acquity UPLC System (Waters)
- Diode array UV/visible detector (Waters)
- Qualified Empower system (Waters)
Solid Phase Extraction (SPE):
1 TomTec Quadra-96 workstation
Radioactivity - liquid scintillation:
1 Tri-Carb 2100 TR (Packard)
1 TopCount NXT (Packard)
Fluorescence/Absorbance/Luminescence:
2 Fluostar Optima BMG microplate readers
Physicochemistry
- Binding to human albumin by Chiral column. HSA as stationary phase (UV-detection)
- Solubility screening by HPLC (UV or MS detection) or nephelometry
- LogP/LogD Screening by Gradient HPLC according to S. Demare, B. Slater, G. Lacome, D. Breuzin, C. Dini, Accurate automated logPo/w Measurement by Gradient-Flow Liquid -Liquid partition Chromatography. Part 1. Neutral Compounds, J. Chromatogr. A, 2007, 1175, p16
